ECL for Chemiluminescent ELISA

NaQ™ ECL Substrate Kit #716

基因名：
货号：
716

Information

Aliases:


Background:
Enhanced chemiluminescence (ECL) reagents are used with horseradish peroxidase (HRP)-conjugated secondary antibodies to detect protein abundance in Western blot. HRP catalyzes luminol oxidation in the presence of hydrogen peroxide, producing light that can be detected by X-ray film and digital imagers. Our NaQ™ reagents are formulated with proprietary enhancers that greatly increase the intensity and duration of emitted light. Our NaQ™ ECL reagents are suitable to detect proteins within the high picogram to low nanogram range. When using NaQ™ ECL reagents, the recommended dilutions for primary and secondary antibodies are 1:1,000-2,000 and 1:10,000-20,000, respectively. Compared to other commonly used ECL reagents, ours exhibit excellent performance by generating intense signal with low background and require less primary and secondary antibodies.


Storage
Stable at 4°C for at least 1 year.


Instructions
Allow the ECL reagents to equilibrate to room temperature approximately 30 min before use.
Wash the membrane thoroughly to reduce non-specific signals.
Mix Reagent A and B at a 1:1 ratio. Vortex to mix.
Immerse the membrane in the mixed solution for 2–3 min.
Drain the excess solution using a paper towel and wrap the membrane in plastic wrap or a clear plastic folder.
Detect the chemiluminescence using X-ray film or a digital imager.


Size Price
200 mL ¥ 2268.00
100 mL ¥ 1308.00
50 mL ¥ 708.00
Datasheet
MSDS

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Note

Product Datasheet
For research use only

BETTER ROOTS BETTER FRUITS

NaQ™ ECL Substrate Kit #716
ECL for Chemiluminescent ELISA


Aliases


                                    


                                    
Background

                                    
Enhanced chemiluminescence (ECL) reagents are used with horseradish peroxidase (HRP)-conjugated secondary antibodies to detect protein abundance in Western blot. HRP catalyzes luminol oxidation in the presence of hydrogen peroxide, producing light that can be detected by X-ray film and digital imagers.
Our NaQ™ reagents are formulated with proprietary enhancers that greatly increase the intensity and duration of emitted light. Our NaQ™ ECL reagents are suitable to detect proteins within the high picogram to low nanogram range. When using NaQ™ ECL reagents, the recommended dilutions for primary and secondary antibodies are 1:1,000-2,000 and 1:10,000-20,000, respectively. Compared to other commonly used ECL reagents, ours exhibit excellent performance by generating intense signal with low background and require less primary and secondary antibodies.

                                    


                                    
Application Information

                                    

                                    


                                    
Immunogen

                                    

                                    


                                    
Isotype

                                    

                                    


                                    
Storage Buffer

                                    

                                    


                                    
Storage

                                    
Stable at 4°C for at least 1 year.

                                    


                                    
Recommended Dilutions

                                    

                                    


                                    
Protocols

                                    

                                

                            

                            

                                        

                                            
Antibody Validation

                                            

                                            

                                        


                            

                            
                        

                    

                


    


    

                

                    

                        
GenuIN Biotech LLC

                        
2000 Kraft Drive, Suite 1110, Blacksburg, VA 24060, USA 

                        
Tel: 0551-163635518  Fax: 540.605.9771

                        
E-mail: info@genuinbiotech.com  www.genuinbiotech.com

                    

                    
Material Safety Data Sheet (MSDS) for Antibody

                    

                        
I. Identification:

                        
Catalog Number: 716

                        
Product Name:  NaQ™ ECL Substrate Kit #716

                        
 Manufacturer & Supplier: Hefei SciBen Biotech Co., Ltd

                        
Address:Building 6, 98 Jiulong Road, Hefei, Anhui 230601, China

                        
Tel: 0551-63635518	

                        
Fax: 540.605.9771	

                        
E-mail:support@scibenbio.com


                        
II. Composition/Information on Ingredients:

                        
This product is composed of antibodies in aqueous buffer solution. According to 29 

                        
CFR1910.1200(d), hazardous ingredients at less than < 1% and carcinogens at less than < 0.1% are considered non-hazardous. Any hazardous or carcinogenic ingredients exceeding these criteria are listed below.

                        
This product may contain the following hazardous ingredients.

                        
Ingredient:glycerol

                        
CAS#:56-81-5

                        
Percent: 50%


                        
III. Hazard Identification:

                        
Emergency Overview of Hazardous ingredient:glycerol (CAS# 56-81-5) 

                        
Caution:Avoid contact and inhalation. 

                        
Target Organ: Kidneys.

                        
NFPA Rating:

                        
Health Rating: 1

                        
Flammability Rating: 0

                        
Reactivity Rating:0


                        
 IV. First Aid Measures:

                        
Inhalation:If inhaled, remove to fresh air. If breathing is difficult, get medical attention.

                        
Ingestion: If swallowed and person is conscious, rinse out mouth with water. Get medical attention.

                        
Skin Exposure:In case of contact, wash skin with soap and water.

                        
Eye Exposure:In case of contact with eyes, immediately flush eyes water for at least 5minutes. Get medical attention.


                        
V. Fire Fighting Measures:

                        
Flash Point: Data not available.

                        
Autoignition Temperature: Data not available.

                        
Fire Extinguishing Media:Water spray, dry chemical, foam, or carbon dioxide.

                        
Firefighting:Wear protective clothing and self-contained breathing apparatus to prevent contact with skin and eyes.








                        
VI. Accidental Release Measures:

                        
Absorb liquid with an absorbent material. Transfer contaminated absorbent to a chemical waste container for disposal.


                        
VII. Handling And Storage:

                        
Avoid inhalation and contact with eyes and skin. Avoid prolonged or repeated exposure. Store at –20 °C in tightly closed container.


                        


                        
VIII. Exposure Controls/Personal Protection:

                        
Engineering Controls: Maintain adequate ventilation, eye wash and quick-drench facilities


                        
IX. Physical and Chemical Properties:

                        
Physical State:Colorless liquid.

                        
Odor:Odorless.

                        
Boiling Point:Data not available.

                        
Melting Point:Data not available.

                        
Volatile Organic Compound:Data not available.

                        
Solubility in water:Readily miscible in water.


                        
X. Stability and Reactivity:

                        
Stability:Stable.

                        
Hazardous Decomposition:May form carbon dioxide and carbon monoxide.

                        
Conditions to avoid:Strong oxidizing agents


                        
XI. Toxicological Information:

                        
May cause skin irritation. 


                        
May be toxic if absorbed through skin or ingested. 

                        
May cause eye irritation.

                        
Target Organs: Kidneys

                        
Prolonged exposure may cause nausea, headache, and vomiting.


                        
XII. Ecological Information:

                        
Data not available.


                        
XIII. Disposal Considerations:

                        
Dispose of in accordance with federal, state and local environmental regulations.


                        
XIV. Transport Information:

                        
D.O.T.: This substance is considered non-hazardous for transport.

                        
IATA: This substance is considered non-hazardous for air transport.


                        
XV. Regulatory Information:

                        
EU Regulation/Classification/Labeling Information: Not available for this product.

                        
Chemical Inventory Status: 

                        
SARA Listed Component: None.

                        
TSCA Listed Component: None.

                        
Canada (WHMIS): DSL No, NDSL No.


                        
XVI. Other Information:

                        

                            This product is sold only for research use by personnel familiar with chemicals and who are
                            well trained in good laboratory habits, such as avoiding spills, keeping hands clean at all times
                            and not rubbing eyes with hands while working in the laboratory.
                            This product is sold only in microliter quantities for use in life sciences research. No other use
                            is intended, and any other use may involve substantive hazards.
                            The above information is believed to be correct but does not purport to be all inclusive and
                            shall be used only as a guide for experienced personnel. Hefei SciBen Biotech Co., Ltd shall
                            not be held liable for any damage resulting from the handling of or from contact with the above product. The burden of safe use of this material rests entirely with the user.
                        

                        
Issuing Date: 8/16/2024 Version: 1.0 


                    

                    
GenuIN Biotech LLC provides the information contained herein in good faith but makes no representation as to its comprehensiveness or accuracy. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this product. Individuals receiving the information must exercise their independent judgment in determining its appropriateness for a particular purpose. GenuIN Biotech LLC makes no representations or warranties, either express or implied, including without limitation any warranties of merchantability, fitness for a particular purpose with respect to the information set forth herein or the product to which the information refers. Accordingly, GenuIN Biotech LLC will not be responsible for damages resulting from use of or reliance upon this information.