Recombinant Antibody

Anti-Autophagy Related 12 Rabbit Monoclonal Antibody #2972

基因名：
ATG12
货号：
2972
应用：
Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
物种反应性：
Human
Western blotting analysis using anti-ATG12 antibody (Cat#2972). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG12 antibody (Cat#2972, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ATG12 expression in HepG2 cells using anti-ATG12 antibody (Cat#2972, 1:2,000). Green, isotype control; red, ATG12.
Immunocytochemical staining of HepG2 cells with anti-ATG12 antibody (Cat#2972, 1:1,000) . Nuclei were stained blue with DAPI; ATG12 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.

Information

Aliases:
ATG12; Autophagy Related 12; APG12; APG12L; Ubiquitin-Like Protein ATG12; APG12 Autophagy 12-Like; ATG12 Autophagy Related 12 Homolog (S. Cerevisiae); Apg12 (Autophagy 12, S. Cerevisiae)-Like; APG12 Autophagy 12-Like (S. Cerevisiae); ATG12 Autophagy Related 12 Homolog; Apg12 (Autophagy, Yeast) Homolog; Autophagy-Related Protein 12; APG12-Like; HAPG12; FBR93


Background:
UniProt Entry: O94817; NCBI Gene Entry: 9140


Application Information
Molecular Weight: Predicted, 15 kDa; observed, 55 kDa
Clonality: Rabbit monoclonal antibody
Clone ID: 24GB3425
Species Reactivity: Human
Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)


Immunogen
A synthesized peptide derived from human Apg12(Atg12)


Isotype
Rabbit IgG


Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.


Storage
Store at -20 °C for one year.


Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:1,000


Protocols
For general and specific antibody protocols please visit our website. Read all instructions before using this product.


Size Price
20 μL ¥ 620.00
50 μL ¥ 1200.00
100 μL ¥ 2200.00
Datasheet
MSDS

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Note

Product Datasheet
For research use only

BETTER ROOTS BETTER FRUITS

Anti-Autophagy Related 12 Rabbit Monoclonal Antibody #2972
Recombinant Antibody


Aliases

ATG12; Autophagy Related 12; APG12; APG12L; Ubiquitin-Like Protein ATG12; APG12 Autophagy 12-Like; ATG12 Autophagy Related 12 Homolog (S. Cerevisiae); Apg12 (Autophagy 12, S. Cerevisiae)-Like; APG12 Autophagy 12-Like (S. Cerevisiae); ATG12 Autophagy Related 12 Homolog; Apg12 (Autophagy, Yeast) Homolog; Autophagy-Related Protein 12; APG12-Like; HAPG12; FBR93


Background

UniProt Entry: O94817; NCBI Gene Entry: 9140


Application Information

Molecular Weight: Predicted, 15 kDa; observed, 55 kDa
Clonality: Rabbit monoclonal antibody
Clone ID: 24GB3425
Species Reactivity: Human
Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)


Immunogen

A synthesized peptide derived from human Apg12(Atg12)


Isotype

Rabbit IgG


Storage Buffer

Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.


Storage

Store at -20 °C for one year.


Recommended Dilutions

Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:1,000


Protocols

For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Antibody Validation

Western blotting analysis using anti-ATG12 antibody (Cat#2972). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG12 antibody (Cat#2972, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).

Antibody Validation

Flow cytometric analysis of ATG12 expression in HepG2 cells using anti-ATG12 antibody (Cat#2972, 1:2,000). Green, isotype control; red, ATG12.

Antibody Validation

Immunocytochemical staining of HepG2 cells with anti-ATG12 antibody (Cat#2972, 1:1,000) . Nuclei were stained blue with DAPI; ATG12 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.

GenuIN Biotech LLC

2000 Kraft Drive, Suite 1110, Blacksburg, VA 24060, USA

Tel: 0551-163635518 Fax: 540.605.9771

E-mail: info@genuinbiotech.com www.genuinbiotech.com

Material Safety Data Sheet (MSDS) for Antibody

I. Identification:

Catalog Number: 2972

Product Name: Anti-Autophagy Related 12 Rabbit Monoclonal Antibody #2972

Manufacturer & Supplier: Hefei SciBen Biotech Co., Ltd

Address:Building 6, 98 Jiulong Road, Hefei, Anhui 230601, China

Tel: 0551-63635518

Fax: 540.605.9771

E-mail:support@scibenbio.com

II. Composition/Information on Ingredients:

This product is composed of antibodies in aqueous buffer solution. According to 29

CFR1910.1200(d), hazardous ingredients at less than < 1% and carcinogens at less than < 0.1% are considered non-hazardous. Any hazardous or carcinogenic ingredients exceeding these criteria are listed below.

This product may contain the following hazardous ingredients.

Ingredient:glycerol

CAS#:56-81-5

Percent: 50%

III. Hazard Identification:

Emergency Overview of Hazardous ingredient:glycerol (CAS# 56-81-5)

Caution:Avoid contact and inhalation.

Target Organ: Kidneys.

NFPA Rating:

Health Rating: 1

Flammability Rating: 0

Reactivity Rating:0

IV. First Aid Measures:

Inhalation:If inhaled, remove to fresh air. If breathing is difficult, get medical attention.

Ingestion: If swallowed and person is conscious, rinse out mouth with water. Get medical attention.

Skin Exposure:In case of contact, wash skin with soap and water.

Eye Exposure:In case of contact with eyes, immediately flush eyes water for at least 5minutes. Get medical attention.

V. Fire Fighting Measures:

Flash Point: Data not available.

Autoignition Temperature: Data not available.

Fire Extinguishing Media:Water spray, dry chemical, foam, or carbon dioxide.

Firefighting:Wear protective clothing and self-contained breathing apparatus to prevent contact with skin and eyes.

VI. Accidental Release Measures:

Absorb liquid with an absorbent material. Transfer contaminated absorbent to a chemical waste container for disposal.

VII. Handling And Storage:

Avoid inhalation and contact with eyes and skin. Avoid prolonged or repeated exposure. Store at –20 °C in tightly closed container.


VIII. Exposure Controls/Personal Protection:

Engineering Controls: Maintain adequate ventilation, eye wash and quick-drench facilities

IX. Physical and Chemical Properties:

Physical State:Colorless liquid.

Odor:Odorless.

Boiling Point:Data not available.

Melting Point:Data not available.

Volatile Organic Compound:Data not available.

Solubility in water:Readily miscible in water.

X. Stability and Reactivity:

Stability:Stable.

Hazardous Decomposition:May form carbon dioxide and carbon monoxide.

Conditions to avoid:Strong oxidizing agents

XI. Toxicological Information:

May cause skin irritation.

May be toxic if absorbed through skin or ingested.

May cause eye irritation.

Target Organs: Kidneys

Prolonged exposure may cause nausea, headache, and vomiting.

XII. Ecological Information:

Data not available.

XIII. Disposal Considerations:

Dispose of in accordance with federal, state and local environmental regulations.

XIV. Transport Information:

D.O.T.: This substance is considered non-hazardous for transport.

IATA: This substance is considered non-hazardous for air transport.

XV. Regulatory Information:

EU Regulation/Classification/Labeling Information: Not available for this product.

Chemical Inventory Status:

SARA Listed Component: None.

TSCA Listed Component: None.

Canada (WHMIS): DSL No, NDSL No.

XVI. Other Information:

This product is sold only for research use by personnel familiar with chemicals and who are well trained in good laboratory habits, such as avoiding spills, keeping hands clean at all times and not rubbing eyes with hands while working in the laboratory. This product is sold only in microliter quantities for use in life sciences research. No other use is intended, and any other use may involve substantive hazards. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide for experienced personnel. Hefei SciBen Biotech Co., Ltd shall not be held liable for any damage resulting from the handling of or from contact with the above product. The burden of safe use of this material rests entirely with the user.

Issuing Date: 8/16/2024 Version: 1.0

GenuIN Biotech LLC provides the information contained herein in good faith but makes no representation as to its comprehensiveness or accuracy. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this product. Individuals receiving the information must exercise their independent judgment in determining its appropriateness for a particular purpose. GenuIN Biotech LLC makes no representations or warranties, either express or implied, including without limitation any warranties of merchantability, fitness for a particular purpose with respect to the information set forth herein or the product to which the information refers. Accordingly, GenuIN Biotech LLC will not be responsible for damages resulting from use of or reliance upon this information.